Knock-In Cell line
Service Details
Cell Types | Various cell types including tumor cells, epithelial cells, and stem cells.Click to view the full list of cell lines |
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Service Types | Fluorescent protein knock-in / Tag protein knock-in |
Delivery Standard | Gene knock-in monoclonal cell line: 1 clone (2 vials, 1×10^6 cells per vial) |
Timeline/Pricing | Consult online for details |
Service Advantages
Efficient sgRNA Design
Optimized Donor Design
Hassle-Free Screening
Service Types
Fluorescent Protein Knock-in | -EGFP, Luc, mCherry, and more. |
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Tag Protein Knock-in | -Flag, HA, Myc, HiBiT, and others. |
Precise insertion of specific DNA fragments into targeted genomic loci. | / |
Targeted knock-in of specific DNA fragments into genomic safe harbor regions. | / |
Service Workflow
Advantage and Characteristic
Optimazied Strategy
Optimazied Strategy
Optimazied Strategy
Optimazied Strategy
Genetic Reference Book
Knock-in strategy based on CLASH technology
The CLASH (Cas9-Linked Adaptor Synthesis for Homology-directed repair) technology enables efficient large-scale gene knock-in for cell engineering. This method combines the Cas9 protein and adaptor synthesis, allowing parallel knock-in across various cell types. By providing specific adaptors during the DNA repair process, it significantly enhances homology-directed repair (HDR) efficiency, thereby increasing knock-in success rates. This technology shows great potential in cell engineering and gene editing, especially for complex bioengineering applications requiring multi-gene modifications.
Enhancing CRISPR-mediated homology-directed repair (HDR) efficiency through cell cycle synchronization
This study explores a method to enhance CRISPR-mediated HDR efficiency by synchronizing the cell cycle. Using small molecules to modulate the cell cycle, researchers achieved a 1.2- to 1.5-fold increase in knock-in efficiency across various cell lines. The study also demonstrated this approach's application in animal embryos, significantly increasing knock-in frequency in pig embryos. This technique improves knock-in success by guiding cells to an HDR-favorable cycle stage, offering a new optimization strategy for CRISPR gene editing.