Overexpression Cell Line

A stable overexpression cell line is created by integrating a target gene into the host cell genome to achieve continuous, long-term overexpression. Stable overexpression cell lines are widely used for gene function studies, disease modeling, drug screening, protein production, gene therapy applications, and more.
Browse Our Overexpression Cell Line Inventory

Service Details

Cell Types Various cell types, including tumor, conventional, stem, primary, and immortalized cell lines.
Services Lentiviral-mediated stable cell lines / Transposon-based stable cell lines
Deliverables Gene overexpression polyclonal cell pool (2 vials per pool, 1×10^6 cells/vial)
Gene overexpression monoclonal cell line ≥1 clone (2 vials/clone, 1×10^6 cells/vial)
Experimental report
Turnaround/Price 4 weeks,as low as $2000   Consult online for details
EDITGENE is dedicated to providing clients with high-quality stable cell line construction services. With years of expertise in cell biology, we have developed a robust and reliable gene overexpression platform. This platform is designed to cater to a wide range of cell types, including hard-to-transfect and suspension cells, offering premium gene overexpression cell line construction services.

EDI-Service Advantages

Versatile Vector Selection
Multiple vector options, including plasmids, lentiviral vectors, and transposons, with customizable promoters, fluorescent tags, and resistance genes.
Efficient Transfection
A well-established transfection system offering various options, including lentiviral transduction, plasmid transfection, and RNP delivery.
Expert Design
Custom vector and strategy design tailored to experimental needs.
Precise Clone Screening
3D printing technology ensures accurate and efficient positive clone selection.
Gold-Standard Validation
Western blotting as the industry benchmark for reliable verification.
Experienced Team
A seasoned team with over 1000 gene editing projects                                                          

Service Types

Customized stable cell line overexpression strategies can be designed and constructed based on client requirements and specific gene characteristics.
Lentiviral Stable Cell Lines Enables stable expression of target genes ≤5kb in size within cells.
Transposon-Based Stable Cell Lines Enables stable expression of target genes ≤10kb in size within cells.

Workflow

Case Study

① Overexpression of CopGFP in A549 cells
1)Vector Map
 
 
 
 
 
2)Overexpression Results
 
Bright Field Image
 
 
GFP Fluorescence Image
 
 
② Overexpression of CopGFP in HCT116 cells
1)Vector Map
 
 
 
 
2)Overexpression Results
 
Bright Field Image
 
 
GFP Fluorescence Image

Advantage and Characteristic

Optimazied Strategy
We have create a unique sgRNA Design Logic
Optimazied Strategy
We have create a unique sgRNA Design Logic
Optimazied Strategy
We have create a unique sgRNA Design Logic
Optimazied Strategy
We have create a unique sgRNA Design Logic

Reference Materials

Article Title: High Expression of SRSF10 Promotes Colorectal Cancer Progression by Aberrant Alternative Splicing of RFC5

This study investigates the role and pathogenesis of serine- and arginine-rich splicing factor 10 (SRSF10) in colorectal cancer (CRC). Bioinformatics analysis predicted SRSF10 gene expression in CRC patients, and functional experiments involving SRSF10 knockdown and overexpression were conducted using CCK8, Transwell, scratch assays, and flow cytometry. SRSF10 knockdown inhibited CRC cell proliferation and migration, promoted apoptosis, and altered DNA replication, while SRSF10 overexpression enhanced proliferation and migration and caused cell cycle changes. Notably, high SRSF10 expression reduced glucose uptake in cultured cells, further indicating its role as a negative regulator of energy metabolism. The study also revealed changes in the RFC5 gene in CRC cells after SRSF10 knockdown, with SRSF10 increasing the exon 2-AS1(S) transcript variant of RFC5, promoting abnormal splicing of exon 2 and advancing CRC progression. These findings suggest that SRSF10 may serve as an important target for CRC clinical diagnosis and treatment.

Article Title: The alteration of LBX1 expression is associated with changes in parameters related to energy metabolism in mice

The LBX1 gene, located near single nucleotide polymorphisms highly associated with adolescent idiopathic scoliosis susceptibility, is considered one of the strongest candidate genes for the disease mechanism. The study found that LBX1 deletion not only causes spinal deformities but also affects lean body mass, suggesting LBX1's potential role in energy metabolism. This study aimed to test this hypothesis by analyzing the phenotype of LBX1-deficient skeletal muscle in mice, focusing on energy metabolism. Results showed that LBX1-deficient mice exhibited greater resistance to high-fat diet-induced obesity, despite similar food intake to control mice. Mutant mice also demonstrated better glucose tolerance, higher maximal aerobic capacity, and increased core temperature. Furthermore, LBX1 overexpression reduced glucose uptake in cultured cells. Together, the data indicate that LBX1 acts as a negative regulator of energy metabolism, with its deletion increasing systemic energy expenditure, leading to lean body mass. The study also suggests a potential association between LBX1 dysfunction and lean body mass in adolescent idiopathic scoliosis patients.

Article Title: Vasorin (VASN) overexpression promotes pulmonary metastasis and resistance to adjuvant chemotherapy in patients with locally advanced rectal cancer

This study examines the role of VASN in rectal cancer, finding that its high expression is associated with pulmonary metastasis and chemotherapy resistance. By establishing stable overexpression and knockdown cell lines of VASN, the authors further validated VASN's role in promoting cancer cell migration, invasion, and proliferation. Both in vitro and in vivo results show that VASN overexpression significantly enhances cancer cell invasion and reduces sensitivity to chemotherapeutic agents. The experimental methods include transfection of the VASN gene using lentiviral vectors, establishment of stable cell lines through G418 resistance selection, and verification of VASN overexpression via Western blot and qPCR.

Selected Customer Resources

IF=50.5
Nature

Abstract:

To date, more than half of global hepatocellular carcinoma (HCC) cases occur in China, yet comprehensive whole-genome analyses focusing on HBV-related HCC within the Chinese population remain scarce. To address this challenge, researchers initiated the China Liver Cancer Atlas (CLCA) project, aiming to conduct large-scale whole-genome sequencing to unravel the unique pathogenic mechanisms and evolutionary trajectories of HCC in China.

The researchers performed deep whole-genome sequencing on 494 HCC tumor samples, with an average depth of 120×, alongside matched blood controls, providing a detailed genomic landscape of HBV-associated HCC. Beyond confirming well-known coding driver genes such as TP53 and CTNNB1, the study identified six novel coding drivers—including FGA—and 31 non-coding driver genes.

Additionally, the research uncovered five new mutational signatures, including SBS_H8, and characterized the presence of extrachromosomal circular DNA (ecDNA) formed via HBV integration, which contributes to oncogene amplification and overexpression. Functional validation experiments demonstrated that mutations in genes such as FGA, PPP1R12B, and KCNJ12 significantly enhance HCC cell proliferation, migration, and invasion.

These findings not only deepen our insights into the genomics of HCC, but also open up new potential targets for diagnosis and therapy. View details>>

Candidate driver landscape

 

IF=27.4
Advanced Materials

Abstract:

During the acute inflammatory phase of tendon injury, excessive activation of macrophages leads to the overexpression of SPP1, which encodes osteopontin (OPN), thereby impairing tissue regeneration. The CRISPR-Cas13 system holds great promise for tissue repair due to its unique RNA editing and rapid degradation capabilities; however, its application has been limited by the lack of efficient delivery methods.

To address this, the researchers systematically screened various cationic polymers targeting macrophages and developed a nanocluster carrier capable of efficiently delivering Cas13 ribonucleoprotein complexes (Cas13 RNPs) into macrophages. Utilizing a reactive oxygen species (ROS)-responsive release mechanism, this system specifically suppresses the overexpression of SPP1 in macrophages within the acute inflammatory microenvironment of tendon injury.

Experimental results demonstrated that this targeted delivery strategy significantly reduced the population of SPP1-overexpressing macrophages induced by injury, inhibited fibroblast activation, and alleviated peritendinous adhesion formation. Furthermore, the study elucidated that SPP1 promotes fibroblast activation and migration through the CD44/AKT signaling pathway, and that inhibiting this pathway effectively mitigates adhesion formation following tendon injury. View details>>

Schematic diagram illustrating immune microenvironment-activated mRNA editing strategies of macrophages for PA therapy

IF=12.8
Biomaterials

Abstract:

Spinal cord injury (SCI) is a severe disabling condition that causes permanent loss of sensory, autonomic, and motor functions. While stem cell therapies, particularly mesenchymal stem cells (MSCs), show great promise for SCI treatment, their limited regenerative capacity restricts their application in tissue repair. The researchers observed that extracellular vesicles derived from antler bud progenitor cells (EVsABPC) may carry bioactive signals that promote tissue regeneration. Accordingly, they isolated and engineered EVs from ABPCs for SCI therapeutic investigation.

The study found that EVsABPC significantly enhanced neural stem cell (NSC) proliferation, promoted axonal growth, reduced neuronal apoptosis, and modulated inflammation by shifting macrophage polarization from the pro-inflammatory M1 phenotype to the anti-inflammatory M2 phenotype. Moreover, engineered EVsABPC modified with cell-penetrating peptides demonstrated improved targeting to the SCI lesion site, markedly enhancing neural regeneration and functional motor recovery. These findings highlight EVsABPC as a promising candidate for SCI therapy. View details>>

Graphical abstract

IF=11.3
Journal of Hazardous Materials

Abstract:

S-metolachlor (S-MET) is one of the most widely produced and applied herbicides in China. Owing to its chemical properties, it tends to persist in soil and easily contaminates surface and groundwater through leaching and runoff. This environmental persistence poses a serious threat to plant development and, through the food chain, to human health.

To address the limitations of current detection technologies and meet the growing demand for high-efficiency analytical tools, the researchers employed a mammalian expression system to generate recombinant antibodies targeting S-MET.

Building on the successful expression of these antibodies, they established a sensitive immunoassay for monitoring S-MET residues in various environmental water samples. The icELISA results showed that the recombinant antibodies retained the sensitivity, specificity, and biological activity of the original monoclonal antibodies, delivering accurate and reproducible detection in river water, agricultural runoff, and tap water. View details>>

Graphical abstract

 

IF=10.7
Biosensors and Bioelectronics

Abstract:

MicroRNAs (miRNAs) are a class of small non-coding RNA molecules that regulate gene expression by interacting with the mRNAs of target genes. Given their crucial role in the development and progression of various diseases, miRNAs have emerged as promising biomarkers for clinical diagnostics.

In this study, researchers established a novel detection platform, termed DBmRCA, which combines dumbbell probe-initiated multi-rolling circle amplification with the high-sensitivity signal output of CRISPR/Cas12a. This enzyme-free, isothermal method enables accurate quantification of miRNA within just 30 minutes.

Clinical validation revealed that the expression levels of miR-200a and miR-126 were significantly downregulated in lung cancer tissues, and results from DBmRCA were consistent with those obtained by conventional techniques. With its high sensitivity, rapid turnaround, and simplified workflow, the DBmRCA platform presents a reliable tool for miRNA detection and holds strong promise for early diagnosis and therapeutic monitoring of lung cancer. View details>>

Graphical abstract

FAQ

EDITGENE brings 10 years of CRISPR-based cell editing experience and offers one-on-one support from a team of PhDs from globally recognized institutions.
Gene overexpression refers to using various techniques to significantly increase the expression level of a specific gene in cells or organisms. This is often achieved by introducing additional gene copies or using strong promoters to drive gene expression.
Gene overexpression aids in studying the function of specific genes, revealing their role within the organism. It is also commonly used in drug screening, vaccine development, and protein production. For example, by overexpressing a therapeutic protein, researchers can evaluate its efficacy in disease models.
The main difference lies in the duration and stability of gene expression: Transient cell line – The target gene is expressed temporarily in cells, typically lasting hours to days, and is suitable for short-term experiments. Stable cell line – The target gene is stably integrated into the cell genome, allowing long-term expression, suitable for extended research and production.

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