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Overexpression Cell Line

A stable overexpression cell line is created by integrating a target gene into the host cell genome to achieve continuous, long-term overexpression. Stable overexpression cell lines are widely used for gene function studies, disease modeling, drug screening, protein production, gene therapy applications, and more.

Service Details

Cell Types Various cell types including tumor cells and stem cells, etc.
Click to view the Comprehensive Cell List
Services Lentiviral-mediated stable cell lines / Transposon-based stable cell lines
Deliverables Gene overexpression polyclonal cell pool (2 vials per pool, 1×10^6 cells/vial)
Gene overexpression monoclonal cell line ≥1 clone (2 vials/clone, 1×10^6 cells/vial)
Experimental report
Turnaround/Price   Consult online for details
EDITGENE is dedicated to providing clients with high-quality stable cell line construction services. With years of expertise in cell biology, we have developed a robust and reliable gene overexpression platform. This platform is designed to cater to a wide range of cell types, including hard-to-transfect and suspension cells, offering premium gene overexpression cell line construction services.

EDI-Service Advantages

Versatile Vector Selection
Multiple vector options, including plasmids, lentiviral vectors, and transposons, with customizable promoters, fluorescent tags, and resistance genes.
Efficient Transfection
A well-established transfection system offering various options, including lentiviral transduction, plasmid transfection, and RNP delivery.
Expert Design
Custom vector and strategy design tailored to experimental needs.
Precise Clone Screening
3D printing technology ensures accurate and efficient positive clone selection.
Gold-Standard Validation
Western blotting as the industry benchmark for reliable verification.
Experienced Team
A seasoned team with over 1000 gene editing projects                                                          

Service Types

Customized stable cell line overexpression strategies can be designed and constructed based on client requirements and specific gene characteristics.
Lentiviral Stable Cell Lines Enables stable expression of target genes ≤5kb in size within cells.
Transposon-Based Stable Cell Lines Enables stable expression of target genes ≤10kb in size within cells.

Workflow

Case Study

① Overexpression of CopGFP in A549 cells
1）Vector Map
 
 
 
 
 
2）Overexpression Results
 
Bright Field Image
 
 
GFP Fluorescence Image
 
 
② Overexpression of CopGFP in HCT116 cells
1）Vector Map
 
 
 
 
2）Overexpression Results
 
Bright Field Image
 
 
GFP Fluorescence Image

Advantage and Characteristic

Optimazied Strategy
We have create a unique sgRNA Design Logic
Optimazied Strategy
We have create a unique sgRNA Design Logic
Optimazied Strategy
We have create a unique sgRNA Design Logic
Optimazied Strategy
We have create a unique sgRNA Design Logic

Genetic Reference Book

Overexpression or knockdown of VASN gene in rectal cancer
Article Title: Vasorin (VASN) overexpression promotes pulmonary metastasis and resistance to adjuvant chemotherapy in patients with locally advanced rectal cancer

This study examines the role of VASN in rectal cancer, finding that its high expression is associated with pulmonary metastasis and chemotherapy resistance. By establishing stable overexpression and knockdown cell lines of VASN, the authors further validated VASN's role in promoting cancer cell migration, invasion, and proliferation. Both in vitro and in vivo results show that VASN overexpression significantly enhances cancer cell invasion and reduces sensitivity to chemotherapeutic agents. The experimental methods include transfection of the VASN gene using lentiviral vectors, establishment of stable cell lines through G418 resistance selection, and verification of VASN overexpression via Western blot and qPCR.

Article Title: The alteration of LBX1 expression is associated with changes in parameters related to energy metabolism in mice

The LBX1 gene, located near single nucleotide polymorphisms highly associated with adolescent idiopathic scoliosis susceptibility, is considered one of the strongest candidate genes for the disease mechanism. The study found that LBX1 deletion not only causes spinal deformities but also affects lean body mass, suggesting LBX1's potential role in energy metabolism. This study aimed to test this hypothesis by analyzing the phenotype of LBX1-deficient skeletal muscle in mice, focusing on energy metabolism. Results showed that LBX1-deficient mice exhibited greater resistance to high-fat diet-induced obesity, despite similar food intake to control mice. Mutant mice also demonstrated better glucose tolerance, higher maximal aerobic capacity, and increased core temperature. Furthermore, LBX1 overexpression reduced glucose uptake in cultured cells. Together, the data indicate that LBX1 acts as a negative regulator of energy metabolism, with its deletion increasing systemic energy expenditure, leading to lean body mass. The study also suggests a potential association between LBX1 dysfunction and lean body mass in adolescent idiopathic scoliosis patients.

Article Title: High Expression of SRSF10 Promotes Colorectal Cancer Progression by Aberrant Alternative Splicing of RFC5

This study investigates the role and pathogenesis of serine- and arginine-rich splicing factor 10 (SRSF10) in colorectal cancer (CRC). Bioinformatics analysis predicted SRSF10 gene expression in CRC patients, and functional experiments involving SRSF10 knockdown and overexpression were conducted using CCK8, Transwell, scratch assays, and flow cytometry. SRSF10 knockdown inhibited CRC cell proliferation and migration, promoted apoptosis, and altered DNA replication, while SRSF10 overexpression enhanced proliferation and migration and caused cell cycle changes. Notably, high SRSF10 expression reduced glucose uptake in cultured cells, further indicating its role as a negative regulator of energy metabolism. The study also revealed changes in the RFC5 gene in CRC cells after SRSF10 knockdown, with SRSF10 increasing the exon 2-AS1(S) transcript variant of RFC5, promoting abnormal splicing of exon 2 and advancing CRC progression. These findings suggest that SRSF10 may serve as an important target for CRC clinical diagnosis and treatment.

FAQ

What is the difference between a stable cell line and a transient cell line?
The main difference lies in the duration and stability of gene expression: Transient cell line – The target gene is expressed temporarily in cells, typically lasting hours to days, and is suitable for short-term experiments. Stable cell line – The target gene is stably integrated into the cell genome, allowing long-term expression, suitable for extended research and production.
Gene overexpression aids in studying the function of specific genes, revealing their role within the organism. It is also commonly used in drug screening, vaccine development, and protein production. For example, by overexpressing a therapeutic protein, researchers can evaluate its efficacy in disease models.
Gene overexpression refers to using various techniques to significantly increase the expression level of a specific gene in cells or organisms. This is often achieved by introducing additional gene copies or using strong promoters to drive gene expression.
EDITGENE brings 10 years of CRISPR-based cell editing experience and offers one-on-one support from a team of PhDs from globally recognized institutions.
EDGENE
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