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FAQ
Can this reagent be used alongside conventional antibiotics like penicillin, streptomycin, amphotericin, or gentamicin?
Since this reagent already provides antibiotic functionality, additional antibiotics are not recommended during the treatment to avoid adding unnecessary stress to the cells.
Can MycoE-A and MycoE-B be used simultaneously?
No, simultaneous use of both reagents places excessive stress on cells and increases the likelihood of resistance development. Use the reagents sequentially according to the instructions.
Residual Mycoplasma detected after treatment
In most cases, mycoplasma should be completely eliminated after treatment. If residual contamination persists, increase the working concentration by 50% and repeat the treatment for another cycle.
Cell death or poor cell condition after Mycoplasma elimination
For most cell lines, Mycoplasma elimination does not affect cell health. However, for sensitive cells, it is recommended to back up the cells before treatment. If cell death or poor conditions occur, reduce the working concentration by half and extend the treatment cycle to 28 days.
Can this reagent be used alongside conventional antibiotics like penicillin, streptomycin, amphotericin, or gentamicin?
Since this reagent already provides antibiotic functionality, additional antibiotics are not recommended during the treatment to avoid adding unnecessary stress to the cells.
Can MycoE-A and MycoE-B be used simultaneously?
No, simultaneous use of both reagents places excessive stress on cells and increases the likelihood of resistance development. Use the reagents sequentially according to the instructions.
Residual Mycoplasma detected after treatment
In most cases, mycoplasma should be completely eliminated after treatment. If residual contamination persists, increase the working concentration by 50% and repeat the treatment for another cycle.
Cell death or poor cell condition after Mycoplasma elimination
For most cell lines, Mycoplasma elimination does not affect cell health. However, for sensitive cells, it is recommended to back up the cells before treatment. If cell death or poor conditions occur, reduce the working concentration by half and extend the treatment cycle to 28 days.
Can it be used with conventional antibiotics like penicillin, streptomycin, amphotericin, or gentamicin?
Since this reagent already provides antibiotic functions, additional antibiotics are not recommended during the treatment to reduce stress on cells.
Residual Mycoplasma after treatment
Mycoplasma should be nearly completely eliminated. If contamination persists, increase the working concentration by 50% and repeat the treatment for one cycle.
Cell death or poor cell condition after Mycoplasma elimination
For most cell lines, mycoplasma elimination has no impact on cell health. However, for sensitive cells, it is recommended to create backups before treatment. If cell death or poor conditions occur, reduce the working concentration by half and extend the treatment period to 28 days.
Can it be used with conventional antibiotics like penicillin, streptomycin, amphotericin, or gentamicin?
Since this reagent already provides antibiotic functions, additional antibiotics are not recommended during the treatment to reduce stress on cells.
Residual Mycoplasma after treatment
Mycoplasma should be nearly completely eliminated. If contamination persists, increase the working concentration by 50% and repeat the treatment for one cycle.
Cell death or poor cell condition after Mycoplasma elimination
For most cell lines, mycoplasma elimination has no impact on cell health. However, for sensitive cells, it is recommended to create backups before treatment. If cell death or poor conditions occur, reduce the working concentration by half and extend the treatment period to 28 days.
Can the kit detect Mycoplasma in reagents?
The kit can detect Mycoplasma in common cell culture reagents, such as media and serum, without the need for sample preparation. Simply proceed directly to PCR reaction and gel loading. However, the kit cannot detect Mycoplasma in organic solvents like DMSO or ethanol.
