EDITGENE CO., LTD

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17800 Castleton St. Ste 665. City of Industry. CA 91748
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info@editxor.com
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+1-833-226-3234 (USA Toll-free)
+1-224-345-1927 (USA)
+86-19120102676 (Intl)

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17800 CASTLETON ST STE 665, CITY OF INDUSTRY,CA 91748

China

  Room 501, Building D, International Business Incubator, No.3 Juquan Road, Science City, Huangpu District, Guangzhou, Guangdong, China 510663

USA

  117800 Castleton St. Ste 665 .City of Industry. CA 91748

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Technical Support

Support Center
Beijing Time: Monday to Friday, 8:00 AM - 6:00 PM
Toll-Free (USA): +833-226-3234
Direct Line (USA): +1-224-345-1927
Email: techsupport@editxor.com

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Beijing Time: Monday to Sunday, 8:00AM - 6:00 PM
International Line: +86-19120102676
Email: info@editxor.com

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FAQ

Transfecting suspension cells is generally more challenging. However, due to its superior performance, this product works efficiently not only in adherent cells but also in suspension cells. For example, in Jurkat cells, 48 hours post-transfection, editing efficiency can reach up to 97%, demonstrating the product’s high efficiency in suspension cell transfection and meeting demanding requirements.
The product utilizes advanced biomolecular transfection technology. Compared with the toxicity of traditional chemical transfection methods and the physical stress of electroporation, it shows significant advantages in preserving cell viability.
If gene knockout fails when using this kit, EDITGENE will not charge for the kit. Additionally, the fee you paid for the kit can be directly applied toward EDITGENE’s gene knockout service, ensuring that your gene editing experiments proceed without concerns.
The product has been validated in multiple cell types. The RNP system enters cells and begins functioning within 4 hours post-transfection, and the Cas9 protein is degraded within 24-48 hours. This transient, high-efficiency expression enables gene editing without the need for continuous selection.
This kit has been validated across multiple cell lines. The RNP complex enters cells and begins gene editing within 4 hours post-transfection. Cas9 protein is degraded within 24–48 hours, allowing efficient and transient expression-driven editing without the need for antibiotic or fluorescent selection.
If gene knockout is unsuccessful when using this kit, EDITGENE will waive the cost of the kit. Moreover, the amount you paid can be fully credited toward EDITGENE’s customized gene knockout services.
While suspension cells are generally more difficult to transfect, this kit performs exceptionally well in both adherent and suspension cell types. For example, in Jurkat cells, editing efficiency can reach up to 97% within 48 hours post-transfection, demonstrating the kit’s outstanding performance and suitability for demanding suspension cell applications.
The kit employs advanced biomolecular transfection technology, offering significant advantages over traditional methods. Unlike chemical transfection, which may be cytotoxic, or electroporation, which can subject cells to physical stress, this approach ensures minimal damage while maintaining high efficiency.
The kit can detect Mycoplasma in common cell culture reagents, such as media and serum, without the need for sample preparation. Simply proceed directly to PCR reaction and gel loading. However, the kit cannot detect Mycoplasma in organic solvents like DMSO or ethanol.
Use a freshly opened negative control for retesting. Ensure to change pipette tips during sample loading and prioritize loading the negative control first to avoid cross-contamination between samples.
PCR detection of Mycoplasma is highly sensitive, so avoid cross-contamination between samples. Ensure sterile conditions when handling each sample individually. After sample processing and PCR reaction, allow samples to cool before proceeding to the next step to avoid aerosol contamination.
The kit can detect Mycoplasma in common cell culture reagents, such as media and serum, without the need for sample preparation. Simply proceed directly to PCR reaction and gel loading. However, the kit cannot detect Mycoplasma in organic solvents like DMSO or ethanol.
PCR detection of Mycoplasma is highly sensitive, so avoid cross-contamination between samples. Ensure sterile conditions when handling each sample individually. After sample processing and PCR reaction, allow samples to cool before proceeding to the next step to avoid aerosol contamination.
Use a freshly opened negative control for retesting. Ensure to change pipette tips during sample loading and prioritize loading the negative control first to avoid cross-contamination between samples.
It is recommended to conduct a pre-transfection experiment before the main experiment. Try different transfection methods to find optimal conditions, such as common chemical transfection methods (e.g., lipofection) or physical methods (e.g., electroporation).
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