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crRNA Design & Synthesis
crRNA consists of two essential components: a hairpin structure that binds to the Cas effector protein and a "protospacer" sequence, typically 20 bases long, that specifically targets a gene fragment. Upon binding to the Cas protein and the target gene fragment, a Cas-crRNA–target ternary complex is formed, enabling the Cas protein to cleave the target sequence. The design of crRNA is adaptable to various detection systems and Cas protein types. Cas13a, guided by crRNA, efficiently recognizes and cleaves single-stranded RNA (ssRNA) targets without strict sequence preferences at the target site, whereas Cas12a enzymes specifically target and cleave double-stranded DNA (dsDNA) sequences that require a protospacer adjacent motif (PAM) (5′ TTTV-3′) for cleavage.
Service Details
| Services | 1、Individual crRNA synthesis2、crRNA design and synthesis |
|---|---|
| Deliverables | 1、crRNA dry powder(1OD/2OD)2、Synthesis quality control report |
| Turnaround/Price |
 Consult online for details |
EDITGENE, leveraging its extensive experience from thousands of CRISPR/Cas cell gene editing cases and CRISPR detection kit development, has developed a proprietary crRNA design logic that significantly enhances the sensitivity and cleavage activity of CRISPR detection. This enables EDITGENE to offer crRNA design and synthesis services to a wide range of IVD companies and research customers.
Service Types
Based on the client's needs, including the target and gene specifics, we offer comprehensive crRNA design and synthesis services.
| Individual crRNA Synthesis | Clients provide the pre-designed crRNA sequences, which EDITGENE will synthesize. |
|---|---|
| crRNA Design and Synthesis | Using information provided by the client, such as the target and gene sequences, we offer crRNA design, synthesis, and activity screening services. |
EDI-Service Advantages
Efficient crRNA Design
Proprietary crRNA design logic to achieve high-efficiency detection.
Flexible Delivery
Various formats available to choose from.
Quality Assurance
HPLC grade, ensuring synthesis purity and quality.
Experienced Team
Technicians with high professionalism and rich experience, capable of designing reasonable solutions based on customer needs.
Workflow
Advantage and Characteristic
Optimazied Strategy
We have create a unique sgRNA Design Logic
Optimazied Strategy
We have create a unique sgRNA Design Logic
Optimazied Strategy
We have create a unique sgRNA Design Logic
Optimazied Strategy
We have create a unique sgRNA Design Logic
