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Overexpression Vector Construction

Gene overexpression involves delivering the target gene into host cells, resulting in its elevated expression. Gene overexpression is widely applied in gene function studies, disease model development, drug screening, protein production, and gene therapy.

Service Details

Services Lentiviral Stable Overexpression Vector Construction / Transposon-Based Stable Overexpression Vector Construction / Transient Overexpression Vector Construction / Custom Vector Construction for Special Requirements
Deliverables 1. Plasmid map
2. Plasmid sequencing results
3. Plasmid amplification instructions
4. Plasmid
Turnaround/Price   Consult online for details
Based on years of experience in cell biology, EDITGENE has developed a mature system for constructing gene overexpression vectors. We offer both stable and transient overexpression vector construction services. Using lentiviral transduction, we can efficiently integrate exogenous genes of up to 5 kb into the host cell genome, ensuring stable expression even after multiple passages. Additionally, for genes larger than 5 kb, we utilize transposon-based methods to achieve long-term stable expression in cells

EDI-Service Advantages

Our lentiviral packaging system enables the stable integration of target genes into the genome of host cells, providing a more reliable tool for gene editing.                                  
Our optimized transposon system offers highly efficient delivery capabilities and a larger payload capacity, ideal for long-term stable expression of larger genes.

Service Types

Customized gene overexpression vectors can be designed and constructed based on client requirements and specific gene characteristics.
1 Lentiviral Stable Overexpression Vector Construction
2 Transposon-Based Stable Overexpression Vector Construction
3 Transient Overexpression Vector Construction
4 Custom Vector Construction for Special Requirements

Plasmid Map

 
Lenti Overexpression Lentiviral Plasmid Map
 
 
 
pPB Overexpression Transposon Plasmid Map

Advantage and Characteristic

Optimazied Strategy
We have create a unique sgRNA Design Logic
Optimazied Strategy
We have create a unique sgRNA Design Logic
Optimazied Strategy
We have create a unique sgRNA Design Logic
Optimazied Strategy
We have create a unique sgRNA Design Logic

Genetic Reference Book

Overexpression of lnc-CTSLP8 in SKOV3 and OVCA420 Cell Lines
Article Title: The lnc-CTSLP8 upregulates CTSL1 as a competitive endogenous RNA and promotes ovarian cancer metastasis

Ovarian cancer is highly lethal and poorly prognostic, primarily due to metastasis. Long non-coding RNAs (lncRNAs) play key roles in tumor progression, but their roles in ovarian cancer metastasis remain unclear. Researchers analyzed the expression of lnc-CTSLP8 in ovarian cancer using public databases (TCGA and GEO) and validated it through qRT-PCR. They constructed lnc-CTSLP8 overexpression and knockout cell lines using lentiviral vectors and the CRISPR/Cas9 system to analyze cell proliferation, migration, and invasion. In vivo studies used an ovarian orthotopic tumor mouse model to observe autophagy and EMT markers in cells, and RNA immunoprecipitation and dual-luciferase reporter gene experiments confirmed the interaction between lnc-CTSLP8 and miR-199a-5p. The results showed high expression of lnc-CTSLP8 in metastatic ovarian cancer, and its overexpression promoted ovarian cancer progression while enhancing autophagy and EMT. Mechanistically, lnc-CTSLP8 upregulates CTSL1 as a competitive endogenous RNA, exhibiting oncogenic effects. CTSL1 inhibitors and miR-199a-5p overexpression eliminated the effects of lnc-CTSLP8 overexpression. The study indicates that lnc-CTSLP8 acts as a ceRNA in ovarian cancer and is a potential therapeutic target.

FAQ

What host bacteria are used for vector construction in EDITGENE? What type of strains can customers use to amplify plasmid vectors?
During vector amplification, Escherichia coli (E. coli) strains are typically used. The commonly used strain for most non-recombinant vectors is DH5α, which is suitable for most applications. For recombinant vectors, such as lentiviral vectors and transposon vectors, the Stbl3 strain can be used for amplification. Stbl3 is a specialized E. coli strain derived from HB101, which has a mutation in the recombinase gene recA13, effectively suppressing recombination of long fragment terminal repeat regions and reducing the likelihood of erroneous recombination.
When selecting a vector, consider the purpose of the experiment and the type of host cells. For example, plasmid vectors are commonly used for gene expression or amplification in bacteria, while viral vectors are more suitable for gene transfer in mammalian cells. Additionally, the vector's promoter, replicon, and antibiotic selection markers should be chosen based on specific requirements.
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