Overexpression Vector Construction
Service Details
Service Types | Lentiviral Stable Overexpression Vector Construction / Transposon-Based Stable Overexpression Vector Construction / Transient Overexpression Vector Construction / Custom Vector Construction for Special Requirements |
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Delivery Standards | 1.Plasmid map2.Plasmid sequencing results3.Plasmid amplification instructions4.Plasmid |
Timeline/Pricing | Consult online for details |
Service Advantages
Service Types
1 | Lentiviral Stable Overexpression Vector Construction |
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2 | Transposon-Based Stable Overexpression Vector Construction |
3 | Transient Overexpression Vector Construction |
4 | Custom Vector Construction for Special Requirements |
Plasmid Map
Advantage and Characteristic
Optimazied Strategy
Optimazied Strategy
Optimazied Strategy
Optimazied Strategy
Genetic Reference Book
Overexpression of lnc-CTSLP8 in SKOV3 and OVCA420 Cell Lines
Ovarian cancer is highly lethal and poorly prognostic, primarily due to metastasis. Long non-coding RNAs (lncRNAs) play key roles in tumor progression, but their roles in ovarian cancer metastasis remain unclear. Researchers analyzed the expression of lnc-CTSLP8 in ovarian cancer using public databases (TCGA and GEO) and validated it through qRT-PCR. They constructed lnc-CTSLP8 overexpression and knockout cell lines using lentiviral vectors and the CRISPR/Cas9 system to analyze cell proliferation, migration, and invasion. In vivo studies used an ovarian orthotopic tumor mouse model to observe autophagy and EMT markers in cells, and RNA immunoprecipitation and dual-luciferase reporter gene experiments confirmed the interaction between lnc-CTSLP8 and miR-199a-5p. The results showed high expression of lnc-CTSLP8 in metastatic ovarian cancer, and its overexpression promoted ovarian cancer progression while enhancing autophagy and EMT. Mechanistically, lnc-CTSLP8 upregulates CTSL1 as a competitive endogenous RNA, exhibiting oncogenic effects. CTSL1 inhibitors and miR-199a-5p overexpression eliminated the effects of lnc-CTSLP8 overexpression. The study indicates that lnc-CTSLP8 acts as a ceRNA in ovarian cancer and is a potential therapeutic target.