Description

This kit is based on rapid nucleic acid amplification technology that functions under isothermal conditions at a moderate temperature (typically 39-42 °C). With the assistance of accessory proteins and single-stranded binding proteins, recombinase forms a complex with the primers, which then searches for and binds to the homologous region of the target sequence. A D-loop structure is formed at the homologous site, initiating strand invasion. As the recombinase dissociates from the complex, DNA polymerase binds to the 3’ end of the primer and begins strand extension. Meanwhile, with the involvement of an exonuclease, a sequence-specific molecular probe designed based on the template is introduced. The use of a fluorescence detection system enables real-time monitoring of the amplification process of the target sequence.

Kit Composition

System Configuration

Reaction Conditions

maintain a constant temperature of 39 ºC; collect fluorescence readings from the FAM channel every 30 seconds, ensure that the signal detection channel matches the fluorescent probe design; total reaction time is 20 minutes.

Storage

·Shipping Temperature: Stable temperature of ≤ 20 ºC.
·Storage Conditions: Store at ≤ -20 ºC (±5 ºC) in a constant temperature environment, protected from light, and avoid heavy pressure and repeated freeze-thaw cycles.
·Shelf Life: 14 months.
·Production Date: See the outer packaging.