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DNA Rapid Isothermal Amplification Kit (Fluorescent Type)
The DNA Rapid Isothermal Amplification Kit (Fluorescent Type) is developed based on a room-temperature isothermal nucleic acid rapid amplification te more
Cat.No: EDN-DY01
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Description
The DNA Rapid Isothermal Amplification Kit (Fluorescent Type) is developed based on a room-temperature isothermal nucleic acid rapid amplification technology. It is suitable for laboratory-grade DNA amplification and other detection applications requiring DNA amplification. By incorporating template-specific molecular probes, the kit enables real-time monitoring of the target fragment amplification process using fluorescence detection equipment.
Kit composition
| Component | Volume |
| A buffer | 1.6 mL×1 vial |
| B buffer | 150 μL×1 vial |
| Positive control template | 30 μL×1 vial |
| Positive control primer-probe mix | 70 μL×1 vial |
| Total | 48 rxns |
| User manual | 1 copy |
system configuration
| Reagent | Volume (μL) |
| A buffer | 29.4 |
| forward primer (10 μM) | 2 |
| reverse primer (10 μM) | 2 |
| Probe (10 μM) | 0.6 |
| ddH2O and DNA template | 13.5 |
| B buffer | 2.5 |
| Total Volume | 50 |
Reaction Conditions
maintain a constant temperature of 39 ºC; collect fluorescence readings from the FAM channel every 30 seconds, ensure that the signal detection channel matches the fluorescent probe design; total reaction time is 20 minutes.
Storage
Shipping Temperature: Stable temperature of ≤ 20 ºC.
·Storage Conditions: Store at ≤ -20 ºC (±5 ºC) in a constant temperature environment, protected from light, and avoid heavy pressure and repeated freeze-thaw cycles.
·Shelf Life: 14 months.
·Production Date: See the outer packaging.
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