CRISPR Cas9 Gene Editing - CRISPR Library Screening - Gene Knockout Cell Line - Knock in Cell Line - Cas12a

Pi Day Surprise: Your Gene Knock-In, as Perfect as π!

▌ Discover the Power of HES-KI Technology for Precision Gene Editing

At EDITGENE, we are proud to offer cutting-edge HES-KI (Homologous End-Joining and Knock-In) Technology, revolutionizing gene editing with unparalleled precision and efficiency. Frustrated by low efficiency and high costs in traditional gene knock-in projects? HES-KI is the solution you’ve been waiting for! By combining CRISPR editing with a positive selection mechanism, HES-KI dramatically enhances knock-in efficiencies, achieving up to 68% success rates, far surpassing conventional methods. Whether you're developing disease models, exploring functional genomics, or advancing therapeutic research, HES-KI ensures reliable results and accelerates your research to new heights. Celebrate Pi Day with a special offer — $314 π Cashback on your HES-KI order, and let precision and efficiency come full circle in your projects.

▌ Why Choose HES-KI?

✔ Exceptional Efficiency:

Achieve up to 68% knock-in efficiency using positive selection mechanisms, far exceeding traditional methods and avoiding non-specific insertions.

✔ Consistent Uniformity:

Monoclonal cell lines exhibit consistent expression of the target gene, providing reliable and reproducible results.

✔ Long-Term Stability:

Stable gene expression even after multiple culture passages, ensuring long-term consistency.

✔ Multi-Gene Integration:

Simultaneously integrate multiple genes, such as CAR genes, to target various tumor antigens and minimize tumor escape.

✔ Flexible Tunability:

Adjust target gene expression levels with ease, selecting from a range of essential genes to fit your experimental needs.

▌ Case Studies: Real-World Results with HES-KI Technology

● Case 1: K562 Cells

Objective: Insert EGFP at the C-terminus of the GAPDH gene in K562 cells.

Design:

Results:

▷ Using the HES-KI technique, EGFP was knocked into the GAPDH gene in K562 cells, achieving a polyclonal insertion efficiency of 37% without resistance selection.

▷ Sanger sequencing confirmed precise EGFP insertion at the C-terminus of the GAPDH gene in K562 cells.

▷ There was no significant difference in doubling time between K562 EGFP-KI monoclonal cell lines and wild-type (WT) cell lines, indicating that the EGFP insertion did not affect K562 cell growth.

▷ Different monoclonal K562 EGFP-KI cell lines exhibited no significant variation in EGFP mRNA expression levels, demonstrating good uniformity.

▷ EGFP mRNA expression remained stable after 15 consecutive passages of K562 EGFP-KI monoclonal cell lines.

Images of K562 EGFP-KI cells:

● Case 2: 293T & CHO-K1 Cells

▷ Objective: Insert EGFP at the C-terminus of the GAPDH gene in 293T and CHO-K1 cells.

Design:

Results:

▷ Using the HES-KI technique, EGFP knock-in efficiencies were 68% in 293T polyclonal cells and 55% in CHO-K1 polyclonal cells.

▷ Sanger sequencing confirmed precise EGFP insertion at the C-terminus of the GAPDH gene in both 293T and CHO-K1 cells.

▷ 293T：

▷ CHO-K1：

Images of polyclonal EGFP-KI cells:

▌ Key Applications of HES-KI Technology:

✔ Gene Therapy:

Achieve stable, effective gene integration for enhanced therapeutic efficacy.

✔ Industrial Production:

Ensure consistent and reliable gene expression for high-quality output.

✔ Stem Cell Engineering:

Improve gene-editing efficiency for regenerative medicine.

✔ Disease Modeling:

Create precise cell lines for faster drug discovery.

✔ Functional Genomics:

Uncover gene functions and regulatory elements with precision editing.

⏰Pi Day Special! Unlock exclusive offers and elevate your research with EDITGENE’s HES-KI technology — achieve the perfect gene knock-in, precise as π!

Get Started with HES-KI Today!

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