EDITGENE offers a comprehensive one-stop solution for CRISPR library screening. We provide a range of services including library design, customization, lentivirus packaging, positive and negative selection, and more. Whether you need pooled libraries or arrayed libraries, CRISPR knockout or CRISPR activation/inhibition, EDITGENE has the expertise to deliver high-quality services.

With over a decade of specialization in gene editing, EDITGENE boasts the most comprehensive series of efficient Cas9 stable transformants and a collection of the hottest CRISPR sgRNA library types in the scientific community. Our expert team provides attentive service, and everything is ready from CRISPR library construction to high-throughput library screening. Place your order and start screening right away!

	The Most Comprehensive sgRNA Library Types：Covers various types of CRISPR libraries, including CRISPR knockout (KO), CRISPRa, and CRISPRi.
	The Most Efficient Cas9 Stable Transformants：With over a hundred verified Cas9 stable transformants, we achieve highly efficient screening.
	The Most Comprehensive Professional Team：Our international team of Ph.D. experts provides full-service support, ensuring that the process is user-friendly even for beginners.

CRISPR Library Screening: What is it?

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CRISPR library screening technology is a biological tool based on the CRISPR/Cas9 system, designed for high-throughput gene function research. The origin of this technology can be traced back to the invention of CRISPR/Cas9, a revolutionary gene editing tool initially used for single gene editing. As research progressed, scientists discovered that by integrating appropriate sgRNA sequences, multiple genes could be intervened or edited simultaneously, leading to the birth of CRISPR library screening technology.

Through high-throughput CRISPR sgRNA library screening, high-throughput gene knockout is achieved, obtaining cells with different gene edits, including gain of function (GOF) and loss of function (LOF), and exploring the biological significance of these genes. CRISPR library screening technology has been widely applied in fields such as gene function, disease mechanisms, drug target identification, and tumor immunology. It not only deepens our understanding of biological processes but also promotes continuous breakthroughs and innovations in disease treatment and biotechnology.

Currently, there are two forms of CRISPR library screening: pooled library and arrayed library. EDITGENE provides services for both types of libraries. Although arrayed libraries are becoming increasingly popular, pooled libraries are cost-effective, easy to operate, and suitable for in vivo studies, allowing for more comprehensive genome coverage. Infected cells can be tested after long-term culture. Therefore, pooled libraries are commonly used for high-throughput screening amongst CRISPR libraries.

Service Categories

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For those who want to screen on their own   ✔  Library Plasmids: Ready-to-use sgRNA libraries  or custom libraries are available. ✔  Lentivirus Packaging: Choose from various specifications. Unsure about the amount of virus needed for your library screening experiment? Consult with our experts at 833-2263234 or info@editxor.com ✔  Efficient Cas9 Stable Transformants Screening Choose from hundreds of verified Cas9 stable transformants at your discretion, combined with top industry 3D single-cell printing technology, EDITGENE offers you the most efficient Cas9 stable transformants screening for polyclonal or monoclonal clones.

For those interested in comprehensive services ✔ Functional Gene and Drug Resistance Gene Screening: Our expert team provides full support, offering professional target screening services. ✔ Whole Genome Library Screening: Unlike the common A/B libraries on the market, EDITGENE utilizes an optimized whole genome library that covers the entire genome in one library, making library screening more accurate and efficient! ✔ Sub-pool sub-library Screening: Choose from dozens of popular sub-libraries in the scientific community, covering all fields! ✔ Arrayed Library Customization: High-throughput arrayed CRISPR screening has become a gradually popular method for target discovery. EDITGENE offers cost-effective arrayed library customization services. Contact us to learn more.

Workflow

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sgRNA Library Construction and Quality Control		Construction of High-Editing Efficiency Cas9 Stable Transgenic Cell Lines		Viral Library Packaging
NGS Sequencing and Bioinformatics Analysis		Positive and Negative Functional Screening		Viral Infection of Cells

Case Study

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The results of gene differential analysis indicate that the CRISPR library screening successfully identified the target genes, achieving a favorable signal-to-noise ratio during the screening process.