-CRISPR Gene Editing|CRISPR Library|KO Cells|Knockin Cells|Cas12a|EDITGENE

CRISPR’s Tricks: How Cas Enzymes Capture, Trim, and Integrate Foreign DNA

New work from Jennifer Doudna’s lab could be useful for CRISPR-based technologies that seek to repurpose Cas1–Cas2 for molecular recording and information storage

2024-07-03

CRISPR Screens Should Be Exhaustive, Not Exhausting

Most CRISPR screens to date have been knockout screens; that is, they have relied on guide RNA–directed nucleases to introduce double-stranded breaks at targeted DNA sites. When these breaks are repaired, errors such as frameshift mutations typically result, disabling target gen…

2024-07-03

Base Editors and Prime Editors Begin to Realize Their Clinical Promise

next-generation CRISPR-based therapeutics. A nuclease discovery platform is used to broaden the targetable range of the genome, and a target-specific optimization platform is used to enable highly precise gene editing, including allele-specific editing, while maintaining high effi…