Product Description
The Mycoplasma qPCR Detection Kit is designed for the detection of mycoplasma DNA extracted from cell suspensions using real-time quantitative PCR (qPCR) technology. To broaden the range of detectable species, the primers and probe included in the kit are specifically designed to target a conserved region of the 16S rRNA gene in mycoplasma. The kit is capable of detecting 22 common mycoplasma species found in cell culture, as well as Escherichia coli, Chlamydia pneumoniae, Legionella pneumophila, and Streptococcus pneumoniae, while minimizing cross-reactivity. The detection limit is as low as 10 copies.
Product Components
| Components | 50T | 100T |
| Myco-Probe Mix | 50 μL | 100 μL |
| Myco-Primer Mix | 50 μL | 100 μL |
| Positive Control DNA (1×107 Copies/μL) | 20 μL | 40 μL |
| 2×Probe qPCR Mix | 50 μL | 100 μL |
| Nuclease-free Water | 1 mL | 2×1 mL |
| Nuclease-free Water | 50 μL | 100 μL |
Workflow Diagram
Figure 1. Schematic Diagram of the Mycoplasma Detection Workflow
Work Areas
Figure 2. Suggested Division of Experimental Areas
Note: The use of a biosafety cabinet depends on the laboratory environment
Storage
Shipped with ice packs; store at -20°C with a shelf life of 12 months.
