Knockout Vector Construction
Service Details
Services | Knockout Lentiviral Vector / Knockout Adenoviral Vector / Knockout Adeno-Associated Viral (AAV) Vector / Knockout Plasmid |
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Deliverables | Plasmid map / Plasmid sequencing results / Plasmid amplification instructions / Plasmids (3 sgRNAs provided separately) |
Turnaround/Price |
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Knockout Strategies
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Frameshift Knockout
Design sgRNA targeting the 5' coding region of the gene to induce indels that are not in multiples of three, resulting in a frameshift mutation.
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Large Fragment DeletionDesign sgRNA to induce the deletion of large segments within the gene.
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Fragment Deletion
Design sgRNA to delete short segments within the gene, causing frameshift mutations.
EDI-Service Advantages
High-Activity Cas9 Plasmid
Efficient sgRNA Design
Diverse Knockout Plasmid Types
Scientific Design
Delivery Standards
1 | Plasmid map |
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2 | Plasmid sequencing results |
3 | Plasmid amplification protocol |
4 | Plasmids (three sgRNAs provided separately) |
Plasmid Map


Advantage and Characteristic

Optimazied Strategy

Optimazied Strategy

Optimazied Strategy

Optimazied Strategy
Reference Materials
FUT8 Gene Knockout in MIA PaCa-2 and PANC-1 Cells
Pancreatic ductal adenocarcinoma (PDAC) is an extremely malignant tumor, accounting for 90% of all pancreatic cancers. Studies have shown that abnormal glycosylation changes on the surface of cancer cells are positively correlated with tumor progression and metastasis. α1,6-fucosyltransferase (FUT8) is a key enzyme responsible for catalyzing core fucosylation, and its abnormal expression and activation in various malignant tumors are associated with multiple physiological and pathological processes. Although the role of FUT8 in other types of cancer has been observed, its specific molecular mechanisms in PDAC malignancy transformation and potential as a therapeutic target remain unclear.
Researchers used the CRISPR/Cas9 system to knock out the FUT8 gene in MIA PaCa-2 and PANC-1 cells. The migration ability of FUT8-KO cells was evaluated using Transwell migration assays and wound healing assays, while the proliferation ability was assessed using MTT assays and colony formation assays. The expression levels of cancer stem cell markers in FUT8-KO cells were also detected. The results showed that compared to wild-type cells, FUT8-KO cells exhibited significantly reduced migration ability, proliferation, and colony formation, along with decreased expression of cancer stem cell markers. FUT8 knockout cells revealed the important role of FUT8 in pancreatic cancer and indicated that FUT8 may be a potential target for pancreatic cancer treatment, providing new insights for future therapeutic strategies against PDAC.
Selected Customer Resources
Deep whole-genome analysis of 494 hepatocellular carcinomas
Abstract:
To date, more than half of global hepatocellular carcinoma (HCC) cases occur in China, yet comprehensive whole-genome analyses focusing on HBV-related HCC within the Chinese population remain scarce. To address this challenge, researchers initiated the China Liver Cancer Atlas (CLCA) project, aiming to conduct large-scale whole-genome sequencing to unravel the unique pathogenic mechanisms and evolutionary trajectories of HCC in China.
The researchers performed deep whole-genome sequencing on 494 HCC tumor samples, with an average depth of 120×, alongside matched blood controls, providing a detailed genomic landscape of HBV-associated HCC. Beyond confirming well-known coding driver genes such as TP53 and CTNNB1, the study identified six novel coding drivers—including FGA—and 31 non-coding driver genes.
Additionally, the research uncovered five new mutational signatures, including SBS_H8, and characterized the presence of extrachromosomal circular DNA (ecDNA) formed via HBV integration, which contributes to oncogene amplification and overexpression. Functional validation experiments demonstrated that mutations in genes such as FGA, PPP1R12B, and KCNJ12 significantly enhance HCC cell proliferation, migration, and invasion.
These findings not only deepen our insights into the genomics of HCC, but also open up new potential targets for diagnosis and therapy. View details>>
Candidate driver landscape
Targeted Macrophage CRISPR-Cas13 mRNA Editing in Immunotherapy for Tendon Injury
Abstract:
During the acute inflammatory phase of tendon injury, excessive activation of macrophages leads to the overexpression of SPP1, which encodes osteopontin (OPN), thereby impairing tissue regeneration. The CRISPR-Cas13 system holds great promise for tissue repair due to its unique RNA editing and rapid degradation capabilities; however, its application has been limited by the lack of efficient delivery methods.
To address this, the researchers systematically screened various cationic polymers targeting macrophages and developed a nanocluster carrier capable of efficiently delivering Cas13 ribonucleoprotein complexes (Cas13 RNPs) into macrophages. Utilizing a reactive oxygen species (ROS)-responsive release mechanism, this system specifically suppresses the overexpression of SPP1 in macrophages within the acute inflammatory microenvironment of tendon injury.
Experimental results demonstrated that this targeted delivery strategy significantly reduced the population of SPP1-overexpressing macrophages induced by injury, inhibited fibroblast activation, and alleviated peritendinous adhesion formation. Furthermore, the study elucidated that SPP1 promotes fibroblast activation and migration through the CD44/AKT signaling pathway, and that inhibiting this pathway effectively mitigates adhesion formation following tendon injury. View details>>
Schematic diagram illustrating immune microenvironment-activated mRNA editing strategies of macrophages for PA therapy
Electrical stimulation of piezoelectric BaTiO3 coated Ti6Al4V scaffolds promotes anti-inflammatory polarization of macrophage and bone repair via MAPK/JNK inhibition and OXPHOS activation
Abstract:
Spinal cord injury (SCI) is a severe disabling condition that causes permanent loss of sensory, autonomic, and motor functions. While stem cell therapies, particularly mesenchymal stem cells (MSCs), show great promise for SCI treatment, their limited regenerative capacity restricts their application in tissue repair. The researchers observed that extracellular vesicles derived from antler bud progenitor cells (EVsABPC) may carry bioactive signals that promote tissue regeneration. Accordingly, they isolated and engineered EVs from ABPCs for SCI therapeutic investigation.
The study found that EVsABPC significantly enhanced neural stem cell (NSC) proliferation, promoted axonal growth, reduced neuronal apoptosis, and modulated inflammation by shifting macrophage polarization from the pro-inflammatory M1 phenotype to the anti-inflammatory M2 phenotype. Moreover, engineered EVsABPC modified with cell-penetrating peptides demonstrated improved targeting to the SCI lesion site, markedly enhancing neural regeneration and functional motor recovery. These findings highlight EVsABPC as a promising candidate for SCI therapy. View details>>
Graphical abstract
Generation of recombinant antibodies by mammalian expression system for detecting S-metolachlor in environmental waters
Abstract:
S-metolachlor (S-MET) is one of the most widely produced and applied herbicides in China. Owing to its chemical properties, it tends to persist in soil and easily contaminates surface and groundwater through leaching and runoff. This environmental persistence poses a serious threat to plant development and, through the food chain, to human health.
To address the limitations of current detection technologies and meet the growing demand for high-efficiency analytical tools, the researchers employed a mammalian expression system to generate recombinant antibodies targeting S-MET.
Building on the successful expression of these antibodies, they established a sensitive immunoassay for monitoring S-MET residues in various environmental water samples. The icELISA results showed that the recombinant antibodies retained the sensitivity, specificity, and biological activity of the original monoclonal antibodies, delivering accurate and reproducible detection in river water, agricultural runoff, and tap water. View details>>
Graphical abstract
Dumbbell probe initiated multi-rolling circle amplification assisted CRISPR/Cas12a for highly sensitive detection of clinical microRNA
Abstract:
MicroRNAs (miRNAs) are a class of small non-coding RNA molecules that regulate gene expression by interacting with the mRNAs of target genes. Given their crucial role in the development and progression of various diseases, miRNAs have emerged as promising biomarkers for clinical diagnostics.
In this study, researchers established a novel detection platform, termed DBmRCA, which combines dumbbell probe-initiated multi-rolling circle amplification with the high-sensitivity signal output of CRISPR/Cas12a. This enzyme-free, isothermal method enables accurate quantification of miRNA within just 30 minutes.
Clinical validation revealed that the expression levels of miR-200a and miR-126 were significantly downregulated in lung cancer tissues, and results from DBmRCA were consistent with those obtained by conventional techniques. With its high sensitivity, rapid turnaround, and simplified workflow, the DBmRCA platform presents a reliable tool for miRNA detection and holds strong promise for early diagnosis and therapeutic monitoring of lung cancer. View details>>
Graphical abstract