Human Subpooled CRISPRi-v2 Libraries-Membrane Proteins #83976

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Size

1×10^8TU 5×10^8TU 1×10^9TU

数量

产品属性

Prouct Name hCRISPRi-v2, Mitochondria, Trafficking, and Motility (h4), top 5 sgRNAs/gene
Species Human
Library Type CRISPRi Library
Plasmid System Two-Plasmid System
Viral Packaging System Third Generation Viral Packaging System
Target Genes 2418
Number Of Grnas 12955
Non-targeting Control Sgrna 250
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Description

EDITGENE specializes in providing high-quality CRISPR screening services. First, a high-coverage and homogeneous plasmid library is prepared (Gini Index <0.1, coverage rate >99%). The plasmid library is then packaged into lentivirus using a well-established system. Virus supernatant is collected, concentrated, and titrated using a cell-based assay to ensure high infection efficiency. The CRISPR library lentivirus provided by EDITGENE can be directly used to construct a library cell pool, streamlining the process by eliminating steps like plasmid library amplification, plasmid library QC, and viral packaging, thus accelerating the experimental workflow.

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