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crRNA Design & Synthesis
crRNA consists of two structural components: one part forms the hairpin structure that binds to the Cas effector, while the other part is a "protospacer" sequence (typically 20 bases) that specifically recognizes the target gene fragment. The crRNA binds to the Cas protein and the target gene fragment to form a Cas-crRNA-target ternary complex, after which the target sequence is cleaved by the Cas protein. Depending on the detection system and the type of Cas protein, different crRNAs can be designed to meet the detection requirements. For instance, Cas12a is guided by crRNA to specifically recognize and cleave dsDNA targets with a PAM sequence (5' TTTN-3'), while Cas13a, guided by crRNA, recognizes and cleaves ssRNA targets without requiring PAMs for target recognition.
Service Details
| Service Content | 1、Individual crRNA synthesis2、crRNA design and synthesis |
|---|---|
| Delivery Standards | 1、crRNA dry powder(1OD/2OD)2、Synthesis quality control report |
| Timeline/Price |
 Consult online for details |
EDITGENE, leveraging its extensive experience from thousands of CRISPR/Cas cell gene editing cases and CRISPR detection kit development, has developed a proprietary crRNA design logic that significantly enhances the sensitivity and cleavage activity of CRISPR detection. This enables EDITGENE to offer crRNA design and synthesis services to a wide range of IVD companies and research customers.
Service Types
Based on the client's needs, including the target and gene specifics, we offer comprehensive crRNA design and synthesis services.
| Individual crRNA Synthesis | Clients provide the pre-designed crRNA sequences, which EDITGENE will synthesize. |
|---|---|
| crRNA Design and Synthesis | Using information provided by the client, such as the target and gene sequences, we offer crRNA design, synthesis, and activity screening services. |
Service Advantages
Efficient crRNA Design
Proprietary crRNA design logic to achieve high-efficiency detection.
Flexible Delivery
Various formats available to choose from.
Quality Assurance
HPLC grade, ensuring synthesis purity and quality.
Experienced Team
Technicians with high professionalism and rich experience, capable of designing reasonable solutions based on customer needs.
Service Workflow
Advantage and Characteristic
Optimazied Strategy
We have create a unique sgRNA Design Logic
Optimazied Strategy
We have create a unique sgRNA Design Logic
Optimazied Strategy
We have create a unique sgRNA Design Logic
Optimazied Strategy
We have create a unique sgRNA Design Logic
