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Product Information
| Catalog Number | Product Name | Components | |||
| EDY003-Y01 |
Bingo™ CRISPR Point Mutation Cell Line Generation Kit (Basic Version) |
Bingo™ Point Mutation Plasmid |
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EDY003-Y02 |
Bingo™ CRISPR Point Mutation Cell Line Generation Kit (Advanced Version) |
Bingo™ Point Mutation Plasmid | |||
| Cell Lysis Reagents | |||||
| PCR Validation Reagents | |||||
Description
This kit is the Bingo™ CRISPR Point Mutation Cell Line Generation Kit, designed for the precise and efficient preparation of cell lines with targeted gene point mutations (substitutions, insertions, deletions). It provides cells, plasmids, and key reagents necessary for the gene point mutation process, offering researchers a highly efficient and convenient tool for one-stop construction of point mutation cell lines.
To significantly reduce experimental costs, this kit also includes Cell Lysis and PCR Validation products. Genomic sample preparation takes only 20 minutes and allows downstream PCR experiments without purification. The PCR Validation reagents are compatible with residual cell lysis components in crude genomic samples, enabling rapid and accurate identification of targeted mutation cell genotypes.
Kit Composition
l Bingo™ Point Mutation Plasmid
| Component | Specification | Storage Temperature | ||
| Helper Plasmids(500 ng/μL) | BingoTM PE | 50μg | ﹣20℃ | |
| Target Plasmids(200 ng/μL) | BingoTM pegRNA1 | BingoTM gRNA1 |
4 μg/pegRNA, 1.5 μg/gRNA |
﹣20℃ |
| BingoTM pegRNA2 | BingoTM gRNA2 | |||
| BingoTM pegRNA3 | BingoTM gRNA3 | |||
| Positive Control(200ng/μL) | BingoTM pegRNA Control | BingoTM gRNA Control |
4 μg/pegRNA, 1.5 μg/gRNA |
﹣20℃ |
| Cell (optional) | 1×106/tube | Liquid nitrogen | ||
l Cell Lysis Reagents
| Component | Specification | Storage Temperature | |||
| Buffer A | 10 mL | RT | |||
| Buffer B | 200 μL | -20 ℃ | |||
l PCR Validation Reagents
| Component | Specification | Storage Temperature | |||
|
2× High Fidelity Pfu Mix (+Dye) |
1.25 mL |
-20 ℃ |
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Genotyping Primer F |
1 OD Powder |
-20 ℃ | |||
|
Genotyping Primer R |
1 OD Powder | -20 ℃ | |||
Workflow Diagram
Figure 1. Workflow Diagram for Gene Point Mutation
Storage Comditions and Shelf Life
-20°C, valid for 1 year.
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