DNA Rapid Isothermal Amplification Kit (Fluorescent Type) Company | DNA Rapid Isothermal Amplification Kit Price

DNA Rapid Isothermal Amplification Kit (Fluorescent Type)

The DNA Rapid Isothermal Amplification Kit (Fluorescent Type) is developed based on a room-temperature isothermal nucleic acid rapid amplification te more

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Description

The DNA Rapid Isothermal Amplification Kit (Fluorescent Type) is developed based on a room-temperature isothermal nucleic acid rapid amplification technology. It is suitable for laboratory-grade DNA amplification and other detection applications requiring DNA amplification. By incorporating template-specific molecular probes, the kit enables real-time monitoring of the target fragment amplification process using fluorescence detection equipment.

Kit composition

Component	Volume
A buffer	1.6 mL×1 vial
B buffer	150 μL×1 vial
Positive control template	30 μL×1 vial
Positive control primer-probe mix	70 μL×1 vial
Total	48 rxns
User manual	1 copy

system configuration

Reagent	Volume (μL)
A buffer	29.4
forward primer (10 μM)	2
reverse primer (10 μM)	2
Probe (10 μM)	0.6
ddH₂O and DNA template	13.5
B buffer	2.5
Total Volume	50

Reaction Conditions

maintain a constant temperature of 39 ºC; collect fluorescence readings from the FAM channel every 30 seconds, ensure that the signal detection channel matches the fluorescent probe design; total reaction time is 20 minutes.

Storage

Shipping Temperature: Stable temperature of ≤ 20 ºC.
·Storage Conditions: Store at ≤ -20 ºC (±5 ºC) in a constant temperature environment, protected from light, and avoid heavy pressure and repeated freeze-thaw cycles.
·Shelf Life: 14 months.
·Production Date: See the outer packaging.