Bingo™ CRISPR Point Mutation Cell Line Generation Kit (Advanced Version)

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Cat.No: EDY003-Y02

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Product Information 

Catalog Number Product Name Components
EDY003-Y01

Bingo™ CRISPR Point Mutation Cell Line Generation Kit (Basic Version)

Bingo™ Point Mutation Plasmid

 

EDY003-Y02

 

Bingo™ CRISPR Point Mutation Cell Line Generation Kit (Advanced Version)


Bingo™ Point Mutation Plasmid
Cell Lysis Reagents
PCR Validation Reagents

 

 

Description

This kit is the Bingo™ CRISPR Point Mutation Cell Line Generation Kit, designed for the precise and efficient preparation of cell lines with targeted gene point mutations (substitutions, insertions, deletions). It provides cells, plasmids, and key reagents necessary for the gene point mutation process, offering researchers a highly efficient and convenient tool for one-stop construction of point mutation cell lines.

To significantly reduce experimental costs, this kit also includes Cell Lysis and PCR Validation products. Genomic sample preparation takes only 20 minutes and allows downstream PCR experiments without purification. The PCR Validation reagents are compatible with residual cell lysis components in crude genomic samples, enabling rapid and accurate identification of targeted mutation cell genotypes.

 

Kit Composition

Bingo™ Point Mutation Plasmid

Component Specification Storage Temperature
Helper Plasmids(500 ng/μL) BingoTM PE 50μg ﹣20℃
Target Plasmids(200 ng/μL) BingoTM pegRNA1 BingoTM gRNA1

4 μg/pegRNA, 

1.5 μg/gRNA

﹣20℃
BingoTM pegRNA2 BingoTM gRNA2
BingoTM  pegRNA3 BingoTM gRNA3
Positive Control(200ng/μL) BingoTM pegRNA Control BingoTM gRNA Control

4 μg/pegRNA, 1.5 μg/gRNA

﹣20℃
Cell (optional)     1×106/tube Liquid nitrogen

Cell Lysis Reagents

Component Specification Storage Temperature
Buffer A 10 mL RT
Buffer B 200 μL -20

PCR Validation Reagents

Component Specification Storage Temperature

2× High Fidelity Pfu Mix (+Dye)

1.25 mL

-20 ℃

Genotyping Primer F

1 OD Powder

-20 ℃

Genotyping Primer R

 1 OD Powder  -20 ℃

 

 

Workflow Diagram

 

Figure 1. Workflow Diagram for Gene Point Mutation

 

 

Storage Comditions and Shelf Life

-20°C, valid for 1 year.

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