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Description
The Lentiviral Packaging Kit includes optimized packaging plasmids, a control plasmid expressing GFP protein, high-efficiency transfection reagents, and lentiviral concentration reagents. This kit offers compatibility with various plasmid systems, a short packaging cycle, high viral titers, and ease of use, making it ideal for beginners in viral packaging.
Kit Composition
| Component | Storage Condition | 10T | 20T |
| Lentipac-Mix Packaging Plasmid | -20 ℃ | 200 μL | 400 μL |
| GFP Control Plasmid | -20 ℃ | 20 μL | 20 μL |
| Trans-PEI Transfection Reagent | 4 ℃ | 70 μL | 150 μL |
| LentiCon-PEG Viral Concentration Solution | 4 ℃ | 40 mL | 80 mL |
Procedure Flowchart
GFP-based Viral Titer Determination
Pre-Experiment for Transfection of 2V6.11 Cell
Storage
The Lentipac-Mix Packaging Plasmid and GFP Control Plasmid stored at -20 ℃ should be protected from repeated freeze-thaw cycles. Thaw at 4 ℃ before use. If multiple uses are expected, it is recommended to aliquot the plasmids after the initial thawing for storage.
An Instruction Manual
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