Description

This kit is based on rapid nucleic acid amplification technology that functions under isothermal conditions at a moderate temperature (typically 39-42 °C). During the reaction, reverse transcriptase synthesizes a complementary DNA (cDNA) strand using specific DNA primers and an RNA template. With the assistance of accessory proteins and single-stranded binding proteins (SSB), recombinase forms a protein/single-stranded DNA complex (Rec/ssDNA) with the primers, which performs homology searching and binds to the target homologous region. A D-loop structure is then formed at the homologous site, initiating strand exchange. As the recombinase dissociates from the complex, DNA polymerase binds to the 3’ end of the primer and begins strand extension. Simultaneously, relying on the activity of a nuclease, a sequence-specific molecular probe designed based on the template is introduced. Using fluorescence detection equipment, the amplification process of the target fragment can be monitored in real time.

Kit Composition

System Configuration

Reaction Conditions

maintain a constant temperature of 42 ºC; collect fluorescence readings from the FAM channel every 30 seconds, ensure that the signal detection channel matches the fluorescent probe design; total reaction time is 20 minutes.

Storage

Shipping Temperature: Stable temperature of ≤ 20 ºC.
Storage Conditions: Store at ≤ -20 ºC (±5 ºC) in a constant temperature environment, protected from light, and avoid heavy pressure and repeated freeze-thaw cycles.
Shelf Life: 14 months.
Production Date: See the outer packaging.