RNA Isothermal Rapid Amplification Kit (Basic Version)
The RNA Rapid Isothermal Amplification Kit (Basic Version) is developed based on a room-temperature isothermal nucleic acid rapid amplification techn more
Cat.No: EDN-RJ01
Quantity
Description
This kit is based on rapid nucleic acid amplification technology that functions under isothermal conditions at a moderate temperature (typically 39-42 °C). During the reaction, reverse transcriptase synthesizes a complementary DNA (cDNA) strand using a specific DNA primer and an RNA template. With the assistance of accessory proteins and single-stranded binding proteins (SSB), the recombinase forms a complex with the primer (Rec/ssDNA), which performs homology searching and binds to the target region. A D-loop structure is then formed, initiating strand invasion. As the recombinase dissociates, DNA polymerase binds to the 3' end of the primer and extends the DNA strand. This kit is intended for laboratory-scale RNA amplification and other RNA detection applications in research settings.
Kit composition
| Component | Volume |
| A buffer | 1.6 mL×1 vial |
| B buffer | 150 μL×1 vial |
| Total | 48 rxns |
| User manual | 1 copy |
System Configuration
| Reagent | Volume (μL) |
| A buffer | 29.4 |
| forward primer (10 μM) | 2 |
| reverse primer (10 μM) | 2 |
| ddH2O and RNA template | 14.1 |
| B buffer | 2.5 |
| Total Volume | 50 |
Reaction Conditions
42 ºC; 30 minutes.
Storage
Shipping Temperature: Stable temperature of ≤ 20 ºC.
Storage Conditions: Store at ≤ -20 ºC (±5 ºC) in a constant temperature environment, protected from light, and avoid heavy pressure and repeated freeze-thaw cycles.
Shelf Life: 14 months.
Production Date: See the outer packaging.
