Knock-In Cell Line

Gene knock-in (KI) is a precise genome editing technique that involves the targeted insertion of exogenous DNA fragments into specific genomic loci, enabling these sequences to fulfill designated biological functions. This method is instrumental in conferring new functionalities to cells or organisms, or in restoring the normal function of defective genes. It plays a critical role in investigating gene function and regulatory mechanisms, constructing disease models, and facilitating advancements in gene therapy.

服务详情

Cell Types Various cell types including tumor cells and stem cells, etc.
Click to view the Comprehensive Cell List
Services Fluorescent protein knock-in / Tag protein knock-in
Deliverables Gene knock-in monoclonal cell line: 1 clone (2 vials, 1×10^6 cells per vial)
Turnaround/Price   Consult online for details
EDITGENE has developed an innovative and highly efficient gene knock-in technology leveraging an upgraded CRISPR/Cas9 system. With over a decade of gene editing expertise, EDITGENE has optimized gRNA and homology arm design strategies, delivering higher positive rates and broader knock-in site flexibility.
 
Gene Knock-in Technology | EDITGENE

EDI-Service Advantages

Efficient sgRNA Design
Proprietary sgRNA design algorithms ensure high precision and efficiency.
Optimized Donor Design
A scientifically driven Donor design system with extensive experience in various Donor vector designs.
Custom-Tailored for Your Experimental Needs
Multiple gene knock-in strategies available to meet diverse experimental requirements.
Streamlined Cell Screening Solutions
3D printing technology facilitates efficient selection of positive monoclonal clones.

Service Types

Customized knock-in strategies can be designed according to client requirements, incorporating specific gene and cell characteristics.
Fluorescent Protein Knock-in -EGFP, Luc, mCherry, and more.
Tag Protein Knock-in -Flag, HA, Myc, HiBiT, and others.
Precise insertion of specific DNA fragments into targeted genomic loci. /
Targeted knock-in of specific DNA fragments into genomic safe harbor regions. /

Workflow

Knock-in Service Workflow | EDITGENE

Advantage and Characteristic

Optimazied Strategy
We have create a unique sgRNA Design Logic
Optimazied Strategy
We have create a unique sgRNA Design Logic
Optimazied Strategy
We have create a unique sgRNA Design Logic
Optimazied Strategy
We have create a unique sgRNA Design Logic

参考文献

Knock-in strategy based on CLASH technology
Article Title: CLASH enables large-scale parallel knock-in for cell engineering

The CLASH (Cas9-Linked Adaptor Synthesis for Homology-directed repair) technology enables efficient large-scale gene knock-in for cell engineering. This method combines the Cas9 protein and adaptor synthesis, allowing parallel knock-in across various cell types. By providing specific adaptors during the DNA repair process, it significantly enhances homology-directed repair (HDR) efficiency, thereby increasing knock-in success rates. This technology shows great potential in cell engineering and gene editing, especially for complex bioengineering applications requiring multi-gene modifications.

Article Title: Modulation of cell cycle increases CRISPR-mediated homology-directed DNA repair

This study explores a method to enhance CRISPR-mediated HDR efficiency by synchronizing the cell cycle. Using small molecules to modulate the cell cycle, researchers achieved a 1.2- to 1.5-fold increase in knock-in efficiency across various cell lines. The study also demonstrated this approach's application in animal embryos, significantly increasing knock-in frequency in pig embryos. This technique improves knock-in success by guiding cells to an HDR-favorable cycle stage, offering a new optimization strategy for CRISPR gene editing.

FAQ

What role does gene knock-in play in drug development?
Gene knock-in plays a crucial role in drug development. It is used in target validation by introducing specific genes into cell lines or animal models to confirm drug target efficacy. It also aids in establishing disease models, testing drug efficacy and safety in these models, and supporting drug screening through high-throughput screening in knock-in cell lines to identify potential drug candidates. Additionally, gene knock-in helps uncover drug mechanisms, optimize drug structure, and improve dosing strategies, expediting drug development while enhancing efficacy and safety.
Gene knock-in technology involves inserting an exogenous gene sequence into a specific location within the genome for gene function studies or disease treatment. Edigene utilizes advanced gene editing tools, such as the CRISPR/Cas9 system, to guide nucleases to cut the target DNA, and employs homology-directed repair or non-homologous end joining to accurately insert the gene at the desired location, achieving efficient and precise gene knock-in.
EDITGENE’s advantages in gene knock-in technology include: Guaranteed results: With 10 years of CRISPR gene editing experience and a team of PhDs from world-renowned institutions offering one-on-one support. High precision: EDITGENE’s optimized tools reduce off-target effects, enhancing editing accuracy. High efficiency: EDITGENE’s technology platform improves knock-in success rates, accelerating experimental progress. Customized service: Tailored knock-in solutions to meet specific research or therapeutic goals.
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