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Mycoplasma Detection Kit (PCR)_50T

货号 : EDMD-01

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Product Description

Mycoplasma is a group of the smallest and simplest prokaryotic organisms. Mycoplasma infection alters the DNA, RNA, and protein expression of cells, leading to slow cell growth rates, morphological changes, and other pathological alterations, which significantly affect experimental results. Detection and prevention of Mycoplasma contamination is an essential step in cell experiments. This kit works by amplifying the conserved region of the Mycoplasma genome using specific primers. After PCR amplification, the presence of Mycoplasma contamination can be confirmed through electrophoresis. This method is characterized by high sensitivity, strong specificity, and rapid detection.

Product Components

Reagent	Storage Conditions	50T(EDMD-01)	100T(EDMD-02)
MycoD Primer MIX	-20℃	100μL	200 μL
MycoD Control template	-20℃	100 μL	200 μL
MycoD Master Mix	-20℃	750 μL	1.5 mL
MycoD Proteinase K Solution	-20℃	50 μL	100 μL

Workflow Diagram

Figure 1. Schematic Diagram of the Mycoplasma Detection Workflow

Work Areas

Figure 2. Suggested Division of Experimental Areas

Note: The use of a biosafety cabinet depends on the laboratory environment

Storage

Shipped with ice packs; store at -20°C with a shelf life of 12 months.

产品说明书

Mycoplasma Detection Kit (PCR)

FAQ

Bands appear in the negative control

Use a freshly opened negative control for retesting. Ensure to change pipette tips during sample loading and prioritize loading the negative control first to avoid cross-contamination between samples.

Inconsistent results with repeated testing

PCR detection of Mycoplasma is highly sensitive, so avoid cross-contamination between samples. Ensure sterile conditions when handling each sample individually. After sample processing and PCR reaction, allow samples to cool before proceeding to the next step to avoid aerosol contamination.

Can the kit detect Mycoplasma in reagents?

The kit can detect Mycoplasma in common cell culture reagents, such as media and serum, without the need for sample preparation. Simply proceed directly to PCR reaction and gel loading. However, the kit cannot detect Mycoplasma in organic solvents like DMSO or ethanol.