产品属性
Prouct Name | Human Subpooled CRISPRa-v2 Libraries- Membrane Proteins (h6), top 5 sgRNAs/gene |
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Prouct Name | Human Subpooled CRISPRa-v2 Libraries- Membrane Proteins (h6), top 5 sgRNAs/gene |
Species | Human |
Species | Human |
Library Type | CRISPRi Library |
Library Type | CRISPRi Library |
Plasmid System | Two-Vector System |
Plasmid System | Two-Vector System |
Viral Packaging System | Third Generation Viral Packaging System |
Viral Packaging System | Third Generation Viral Packaging System |
Target Genes | 18899 |
Target Genes | 18899 |
Number Of Grnas | 57011 |
Number Of GRNAs | 57011 |
Non-targeting Control Sgrna | 500 |
Non-Targeting Control SgRNA | 500 |
Tag | puro |
TAG | puro |
Description
EDITGENE specializes in providing high-quality CRISPR screening services. First, a high-coverage and homogeneous plasmid library is prepared (Gini Index <0.1, coverage rate >99%). The plasmid library is then packaged into lentivirus using a well-established system. Virus supernatant is collected, concentrated, and titrated using a cell-based assay to ensure high infection efficiency. The CRISPR library lentivirus provided by EDITGENE can be directly used to construct a library cell pool, streamlining the process by eliminating steps like plasmid library amplification, plasmid library QC, and viral packaging, thus accelerating the experimental workflow.
Backbone Map
![](/uploads/20241023/9dkFx5CSoaPIU376_4ab4d59a32742e779212a37c617fc4b8.png)