产品属性
Prouct Name | Human Subpooled CRISPRi-v2 Libraries-Membrane Proteins #83976 |
---|---|
Prouct Name | Human Subpooled CRISPRi-v2 Libraries-Membrane Proteins #83976 |
Species | Human |
Species | Human |
Library Type | CRISPRi Library |
Library Type | CRISPRi Library |
Plasmid System | Two-Vector System |
Plasmid System | Two-Vector System |
Viral Packaging System | Third Generation Viral Packaging System |
Viral Packaging System | Third Generation Viral Packaging System |
Target Genes | 18901 |
Target Genes | 18901 |
Number Of Grnas | 57050 |
Number Of GRNAs | 57050 |
Non-targeting Control Sgrna | 500 |
Non-Targeting Control SgRNA | 500 |
Tag | puro |
TAG | puro |
Description
EDITGENE specializes in providing high-quality CRISPR screening services. First, a high-coverage and homogeneous plasmid library is prepared (Gini Index <0.1, coverage rate >99%). The plasmid library is then packaged into lentivirus using a well-established system. Virus supernatant is collected, concentrated, and titrated using a cell-based assay to ensure high infection efficiency. The CRISPR library lentivirus provided by EDITGENE can be directly used to construct a library cell pool, streamlining the process by eliminating steps like plasmid library amplification, plasmid library QC, and viral packaging, thus accelerating the experimental workflow.
Backbone Map
![](/uploads/20241023/9dkFx5CSoaPIU376_4ab4d59a32742e779212a37c617fc4b8.png)