产品属性
Prouct Name | Yeo Lab RNA-Binding Protein Pooled CRISPR Knockout Library。 |
---|---|
Prouct Name | Yeo Lab RNA-Binding Protein Pooled CRISPR Knockout Library。 |
Species | Human |
Species | Human |
Library Type | CRISPR KO Library |
Library Type | CRISPR KO Library |
Plasmid System | One-Vector System |
Plasmid System | One-Vector System |
Viral Packaging System | Third Generation Viral Packaging System |
Viral Packaging System | Third Generation Viral Packaging System |
Target Genes | 1078 |
Target Genes | 1078 |
Number Of Grnas | 12472 |
Number Of GRNAs | 12472 |
Non-targeting Control Sgrna | 1698 |
Non-Targeting Control SgRNA | 1698 |
Tag | puro |
TAG | puro |
Description
EDITGENE specializes in providing high-quality CRISPR screening services. First, a high-coverage and homogeneous plasmid library is prepared (Gini Index <0.1, coverage rate >99%). The plasmid library is then packaged into lentivirus using a well-established system. Virus supernatant is collected, concentrated, and titrated using a cell-based assay to ensure high infection efficiency. The CRISPR library lentivirus provided by EDITGENE can be directly used to construct a library cell pool, streamlining the process by eliminating steps like plasmid library amplification, plasmid library QC, and viral packaging, thus accelerating the experimental workflow.
Backbone Map
![](/uploads/20241023/XbHZYWwK3te9xa5c_37f57681c4c04ebdf9721c03fddee792.png)