产品属性
Prouct Name | Bassik lab Human CRISPR-Cas9 Deletion Library - Apoptosis and cancer |
---|---|
Prouct Name | Bassik lab Human CRISPR-Cas9 Deletion Library - Apoptosis and cancer |
Species | Human |
Species | Human |
Library Type | CRISPR KO Library |
Library Type | CRISPR KO Library |
Plasmid System | Two-Vector System |
Plasmid System | Two-Vector System |
Viral Packaging System | Third Generation Viral Packaging System |
Viral Packaging System | Third Generation Viral Packaging System |
Target Genes | 3015 |
Target Genes | 3015 |
Number Of Grnas | 31324 |
Number Of GRNAs | 31324 |
Non-targeting Control Sgrna | 1500 |
Non-Targeting Control SgRNA | 1500 |
Tag | puro |
TAG | puro |
Description
EDITGENE specializes in providing high-quality CRISPR screening services. First, a high-coverage and homogeneous plasmid library is prepared (Gini Index <0.1, coverage rate >99%). The plasmid library is then packaged into lentivirus using a well-established system. Virus supernatant is collected, concentrated, and titrated using a cell-based assay to ensure high infection efficiency. The CRISPR library lentivirus provided by EDITGENE can be directly used to construct a library cell pool, streamlining the process by eliminating steps like plasmid library amplification, plasmid library QC, and viral packaging, thus accelerating the experimental workflow.
Backbone Map
![](/uploads/20241023/f6BQtabn15CAXRsW_41f8cd9dc7c0e0f5ee212c660fcb44a3.png)