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DNA Rapid Isothermal Amplification Kit (Fluorescent Type)
The DNA Rapid Isothermal Amplification Kit (Fluorescent Type) is developed based on a room-temperature isothermal nucleic acid rapid amplification technology. It is suitable for laboratory-grade DNA amplification and other detection applications requiring DNA amplification. By incorporating template-specific molecular probes, the kit enables real-time monitoring of the target fragment amplification process using fluorescence detection equipment. 更多
货号 : EDN-DY01
数量
Description
This kit is based on rapid nucleic acid amplification technology that functions under isothermal conditions at a moderate temperature (typically 39-42 °C). With the assistance of accessory proteins and single-stranded binding proteins, recombinase forms a complex with the primers, which then searches for and binds to the homologous region of the target sequence. A D-loop structure is formed at the homologous site, initiating strand invasion. As the recombinase dissociates from the complex, DNA polymerase binds to the 3’ end of the primer and begins strand extension. Meanwhile, with the involvement of an exonuclease, a sequence-specific molecular probe designed based on the template is introduced. The use of a fluorescence detection system enables real-time monitoring of the amplification process of the target sequence.
Kit Composition
| Component | Volume |
| A buffer | 1.6 mL×1 vial |
| B buffer | 150 μL×1 vial |
| Positive control template | 30 μL×1 vial |
| Positive control primer-probe mix | 70 μL×1 vial |
| Total | 48 rxns |
| User manual | 1 copy |
System Configuration
| Reagent | Volume (μL) |
| A buffer | 29.4 |
| forward primer (10 μM) | 2 |
| reverse primer (10 μM) | 2 |
| Probe (10 μM) | 0.6 |
| ddH2O and DNA template | 13.5 |
| B buffer | 2.5 |
| Total Volume | 50 |
Reaction Conditions
maintain a constant temperature of 39 ºC; collect fluorescence readings from the FAM channel every 30 seconds, ensure that the signal detection channel matches the fluorescent probe design; total reaction time is 20 minutes.
Storage
·Shipping Temperature: Stable temperature of ≤ 20 ºC.
·Storage Conditions: Store at ≤ -20 ºC (±5 ºC) in a constant temperature environment, protected from light, and avoid heavy pressure and repeated freeze-thaw cycles.
·Shelf Life: 14 months.
·Production Date: See the outer packaging.
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