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DNA Rapid Isothermal Amplification Kit (Colloidal gold test strip)
The DNA Rapid Isothermal Amplification Kit (Colloidal gold test strip) is developed based on a room-temperature isothermal nucleic acid rapid amplification technology. It is suitable for laboratory-grade DNA amplification and other detection applications requiring DNA amplification. By incorporating template-specific molecular probes, the kit utilizes colloidal gold technology (sandwich immunoassay) to detect the final results. 更多
货号 : EDN-DT01
数量
Description
This kit is based on rapid nucleic acid amplification technology that functions under isothermal conditions at a moderate temperature (typically 39-42 °C). Under the assistance of accessory proteins and single-stranded DNA-binding protein (SSB), recombinase and primers form a Rec/ssDNA complex that performs homology searching and binds to the target homologous region. A D-loop is formed at the homologous site, initiating strand exchange. As the recombinase dissociates from the complex, DNA polymerase binds to the 3′ end of the primer and initiates strand extension. With the action of the endonuclease (nfo), a specific molecular probe designed based on the template is introduced. The final result can be detected using colloidal gold-based sandwich immunoassay technology.
Kit Composition
| Component | Volume |
| A buffer | 1.6 mL×1 vial |
| B buffer | 150 μL×1 vial |
| Positive control template | 30 μL×1 vial |
| Positive control primer-probe mix | 70 μL×1 vial |
| Total | 48 rxns |
| User manual | 1 copy |
System Configuration
| Reagent | Volume (μL) |
| A buffer | 29.4 |
| forward primer (10 μM) | 2 |
| reverse primer (10 μM) | 2 |
| Probe (10 μM) | 0.6 |
| ddH2O and DNA template | 13.5 |
| B buffer | 2.5 |
| Total Volume | 50 |
Reaction Conditions
maintain a constant temperature of 39 ºC; collect fluorescence readings from the FAM channel every 30 seconds, ensure that the signal detection channel matches the fluorescent probe design; total reaction time is 20 minutes.
Storage
·Shipping Temperature: Stable temperature of ≤ 20 ºC.
·Storage Conditions: Store at ≤ -20 ºC (±5 ºC) in a constant temperature environment, protected from light, and avoid heavy pressure and repeated freeze-thaw cycles.
·Shelf Life: 14 months.
·Production Date: See the outer packaging.
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