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DNA Isothermal Amplification Kit (Basic Version)
The DNA Isothermal Amplification Kit (Basic Version) is developed based on a room-temperature isothermal nucleic acid rapid amplification technology. It is suitable for laboratory-grade DNA amplification and other detection applications requiring DNA amplification. 更多
货号 : EDN-DJ01
数量
Description
This kit is based on rapid nucleic acid amplification technology that functions under isothermal conditions at a moderate temperature (typically 39-42 °C). Under the assistance of accessory proteins and single-stranded binding proteins, recombinase forms a complex with primers, which then performs homology searching and binds to the target homologous region. A D-loop structure is formed at the homologous site, initiating strand invasion. As the recombinase dissociates from the complex, DNA polymerase binds to the 3’ end of the primer and begins strand extension.
Kit Composition
| Component | Volume |
| A buffer | 1.6 mL×1 vial |
| B buffer | 150 μL×1 vial |
| Positive control template | 30 μL×1 vial |
| Positive control primer-probe mix | 70 μL×1 vial |
| Total | 48 rxns |
| User manual | 1 copy |
System Configuration
| Reagent | Volume (μL) |
| A buffer | 29.4 |
| forward primer (10 μM) | 2 |
| reverse primer (10 μM) | 2 |
| Probe (10 μM) | 0.6 |
| ddH2O and DNA template | 13.5 |
| B buffer | 2.5 |
| Total Volume | 50 |
Reaction Conditions
maintain a constant temperature of 39 ºC; collect fluorescence readings from the FAM channel every 30 seconds, ensure that the signal detection channel matches the fluorescent probe design; total reaction time is 20 minutes.
Storage
·Shipping Temperature: Stable temperature of ≤ 20 ºC.
·Storage Conditions: Store at ≤ -20 ºC (±5 ºC) in a constant temperature environment, protected from light, and avoid heavy pressure and repeated freeze-thaw cycles.
·Shelf Life: 14 months.
·Production Date: See the outer packaging.
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